 |
The key benefits of the AutoGel software are:
Increased accuracy of results
High speed analyzing
Reduced requirements on technical staff
Autogel was developed to overcome the problems associated with the manual analysis of gel images. Most software solutions rely on human interpretation of images and the assignment of allele values manually. This process is both time consuming and relies heavily on the knowledge of the lab technician.
With modern laboratories generating many images each day, it becomes very taxing on the lab technician and allows for the introduction of human errors. The speed at which images are processed is also a key factor, if you tie up a valuable technical resource for many hours a week it's not economically viable.
Image Processing
Many resulting DNA band images are very poor in quality, images are often fuzzy full of scratches and can have long steaks resulting from the travel of the band through the gel in the electropheresis process. AutoGel has proprietary gel processing algorithms that isolate DNA bands and remove a high proportion of image noise before processing occurs. This ensures the best quality gel image is available to process and generate accurate results.
Band Processing
The processing of band information is essential to the overall allele result, often when multiple lanes are run on the same gel, the lanes run at different rates giving a bowing effect and distorting the position of the alleles. The AutoGel software uses the internal standards and allelic ladders normally run in a gel to repair the image and bring it back to a isometric state. The processing of bands can then be performed accurately and without errors. The band intensity is also accounted for, thus helping reduce the possibility of false or DNA band shadows.
Reporting
The final results are values of alleles on defined regions. These values can be reported directly in a document, saved in a test file or added to a database. The flexibility in the reporting of gels mean that a laboratory can run a large number of gels and have the results exported to any package that processes or stores the results. It is ideal for profiling and disease testing.
| |
